PRÉSENTATION DU PROJET INDIVIDUEL 3
Regulation of the virulon of L. monocytogenes by carbohydrates
Host organisation: University of Copenhagen (UCPH) Secondments: University College Cork (UCC) and University of Burgundy (UB)
ORGANISATION D’ACCUEIL ET SUPERVISEUR:
Université de Copenhague, Dr. Hanne INGMER (plus d’informations)
OBJECTIFS:
Preliminary data suggest that host sugar-molecules have chitin-resembling moieties and that these are substrates for both bacterial and human chitinases. Considering that the L. monocytogenes chitinases are important in both environmental and host adaptation, we wish to identify the host chitin-type molecules and determine the role of these molecules as well as of the listerial chitinases for attachment, invasion and long-term survival in host cells. To assess the environmental role of the L. monocytogenes chitinases we will determine the regulation of chitinase expression and investigate the conditions at which they are expressed. The role of chitinases in adaptability of L. monocytogenes will be determined by investigation of the respective mutants in growth and survival in the various environments and the ability of the mutants to survive and initiate growth after abrupt changes in environments.Regarding the critical role of carbohydrates in regulation, this project will use combinations of proteomic and transcriptomic approaches to dissect the intricate regulatory mechanisms controlling chitinase expression. This will be studied using transposon mutagenesis and site-directed mutagenesis.
RÉSULTATS ATTENDUS:
Global transcriptomic response to chitin. Determination of TSS. Identification of proteins critical for regulation of chitinase expression. Evaluation of the role of chitinases during infection. Contribution of chitinases to soil and plant adaptation
SECONDMENTS:
Université Collège Cork (plus d’informations) et Université de Bourgogne (plus d’informations)
DUREE DU RECRUTEMENT :
36 mois
PRÉSENTATION DU CHERCHEUR
Miguel VILLORIA RECIO
PAYS D’ORIGINE: Espagne
FORMATION :
- Licence en biologie à l’Université de Salamanque, Espagne
- Erasmus à l’Université de Turin, Italie et l’Université d’Édimbourg, Écosse
- Master en recherche sur les maladies infectieuses à l’Université d’Édimbourg, Écosse
CONTACT :
michirecio@sund.ku.dk
https://dk.linkedin.com/in/miguel-villoria-recio-2a387594
(plus d’informations sur la page anglaise)
Ce projet est financé par les actions Marie-Sklodowska Curie du programme de recherche et d’innovation de l’Union européenne Horizon 2020 selon l’accord de subvention n°641984
